Last edited by Bajind
Saturday, August 8, 2020 | History

2 edition of TRG, a chemotactic transducer of E. coli found in the catalog.

TRG, a chemotactic transducer of E. coli

Dawn Marie Nowlin

TRG, a chemotactic transducer of E. coli

analysis of covalent modifications and homology to related proteins

by Dawn Marie Nowlin

  • 24 Want to read
  • 35 Currently reading

Published .
Written in English

    Subjects:
  • Escherichia coli.

  • Edition Notes

    Statementby Dawn Marie Nowlin.
    The Physical Object
    Paginationx, 173 leaves, bound :
    Number of Pages173
    ID Numbers
    Open LibraryOL16596387M

    a transducer protein of unknown specificity. Introduction Bacterial chemotaxis is the process by which bacteria migrate purposefully through concentration gradients of certain substances. In Escherichia coli and Salmo- nella typhimurium, chemotactic behavior stems from regulation of . In Escherichia coli, methylation and demethylation of four related membrane proteins, the methyl-accepting chemotaxis proteins (or MCPs), is central to chemotactic sensing and signal transduction.

    Cloning of trg, a gene for a sensory transducer in Escherichia coli Article (PDF Available) in Journal of Bacteriology (1) November with 20 Reads How we measure 'reads'. Chemotactic behavior. Chemotaxis, movement toward or away from chemicals, is a universal attribute of motile cells and organisms. E. coli cells swim toward amino acids (serine and aspartic acid), sugars (maltose, ribose, galactose, glucose), dipeptides, pyrimidines and electron acceptors (oxygen, nitrate, fumarate). Figure 1 shows two simple methods for assessing attractant responses by E. coli.

    The methyl-accepting chemotactic-signal transducers of the enteric bacteria are transmembrane proteins that consist of a periplasmic receptor domain and a cytoplasmic signaling domain. To study their evolution, transducer genes from Enterobacter aerogenes and Klebsiella pneumoniae were compared with transducer genes from Escherichia coli and. "Aspartate and maltose-binding protein interact with adjacent sites in the Tar chemotactic signal transducer of Escherichia coli." J Bacteriol (5); PMID: Gardina Gardina PJ, Manson MD (). "Attractant signaling by an aspartate chemoreceptor dimer with a single cytoplasmic domain." Science (); PMID:


Share this book
You might also like
Signing

Signing

Embroidered with white

Embroidered with white

Public-private competitions

Public-private competitions

Baba

Baba

Divided Line and Dianoetic Eikasia or the art of weaving as exegetical devices for some Platonic dialogues

Divided Line and Dianoetic Eikasia or the art of weaving as exegetical devices for some Platonic dialogues

Memoirs of a paid mourner

Memoirs of a paid mourner

return of Jesus ...

return of Jesus ...

Hudson drawing tables

Hudson drawing tables

India and the EEC

India and the EEC

Glen More, Cairngorms

Glen More, Cairngorms

Nocturne, op. 9, no. 2.

Nocturne, op. 9, no. 2.

Cariboo Chilcotin, British Columbia, Canada

Cariboo Chilcotin, British Columbia, Canada

A nickel jackpot

A nickel jackpot

Mindwaves

Mindwaves

TRG, a chemotactic transducer of E. coli by Dawn Marie Nowlin Download PDF EPUB FB2

Transducers are transmembrane proteins that are central to the chemotactic system of Escherichia coli. The proteins transduce ligand recognition into an excitatory signal and function in adaptation as methyl-accepting proteins.

The transducer genes tsr, tar, Cited by: 5. Berg HC, Tedesco PM. Transient response to chemotactic stimuli in Escherichia coli. Proc Natl Acad Sci U S A. Aug; 72 (8)– [PMC free article] Bitner RM, Kuempel PL. P1 transduction mapping of the trg locus in rac+ and rac strains of Escherichia coli K J Bacteriol.

Feb; (2)– [PMC free article]Cited by: Gardina P, Conway C, Kossman M, Manson M. Aspartate and maltose-binding protein interact with adjacent sites in the Tar chemotactic signal transducer of Escherichia coli. J Bacteriol. Mar; (5)– [PMC free article] Groarke JM, Mahoney WC, Hope JN, Cited by: Transducers are transmembrane proteins that are central to the chemotactic system of Escherichia coli.

The proteins transduce ligand recognition into an excitatory signal and function in. Nowlin DM, Nettleton DO, Ordal GW, Hazelbauer GL. Chemotactic transducer proteins of Escherichia coli exhibit homology with methyl-accepting proteins from distantly related bacteria. J Bacteriol. Jul; (1)– [PMC free article] Porcella SF, Popova TG, Akins DR, Li M, Radolf JD, Norgard by: Chemotactic TransducerProteins ofEscherichia coli Exhibit For the transducers of E.

coli, the tryptic peptides containing the methyl-accepting sites of each protein are Immunoprecipitation ofmethyl-accepting chemotaxis proteins ofB. subtilis and S. aurantia by an antiserum raised to the Trg transducer of E. coli. Escherichia coli exhibits chemotactic responses to sugars, amino acids, and dipeptides, and the responses are mediated by methyl-accepting chemotaxis proteins (MCPs).

Using capillary assays, we demonstrated that Escherichia coli RP is attracted to the pyrimidines thymine and uracil and the response was constitutively expressed under all tested growth conditions.

Purification of receptor protein Trg by exploiting a property common to chemotactic transducers of Escherichia coli. J Biol Chem. Oct 15; (29)– Chelsky D, Dahlquist FW.

Structural studies of methyl-accepting chemotaxis proteins of Escherichia coli:. Escherichia coli exhibits chemotactic responses to sugars, amino acids, and dipeptides, and the responses are mediated by methyl-accepting chemotaxis proteins (MCPs).

Using capillary assays, we demonstrated that Escherichia coli RP is attracted to the pyrimidines thymine and uracil and the response was constitutively. Ames, P. & Parkinson, J. Transmembrane signaling by bacterial chemoreceptors: E.

coli transducers with locked signal output. C – (). CAS Google Scholar. CHEMOTACTIC-SIGNAL TRANSDUCERS IN ENTERIC BACTERIA Ciat pRK41 EcoRI pMK1 Clal NdeI I I NruI Nrul tar ( him.) Tetr AvaI NdeI NdeI l CIal NdeI 6,8kb EcoRI ori ^,Apr o= C== EcoRI kb CIalI pMD6 ori Ampr *=C-Z Aval Ndel NdeI I I tor (SE) CIal.

Bollinger J, Park C, Harayama S, Hazelbauer GL. Structure of the Trg protein: Homologies with and differences from other sensory transducers of Escherichia coli. Proc Natl Acad Sci U S A.

Jun; 81 (11)– [PMC free article] Boyd A, Kendall K, Simon MI. Structure of the serine chemoreceptor in Escherichia coli. Nature. A truncated trg gene was created that coded for the carboxyl-terminal three-fifths of the transducer, which constitutes the cytoplasmic domain common to all four transducers in E.

coli. In Escherichia coli this class of proteins is exemplified by the sensory transducers (also called signalling proteins or methyl-accepting chemotaxis proteins) which have a central role in.

Bacterial strains and plasmids. CP and RP are strains of E. coli KThe former carries chromosomal deletions of trg, tsr,tar, tap, cheR, and latter, provided by J.

Parkinson (University of Utah), carries a deletion from flhA through flhD and thus lacks the genes for all Che proteins. pGB1 and pNT carry trg and tar, respectively, under the control of a tac promoter. Results Isolation of Locked Transducer Mutants There are four MCP species in E. coli (Tar, Tap, Trg, and Tsr), each of which mediates responses to a different set of chemoeffector compounds.

Mutations that inactivate one of these transducers eliminate only a subset of chemotactic responses (Harayama et al., ; Parkinson, ; Slocum and. Chemotactic transducer proteins of Escherichia coli exhibit homology with methyl-accepting proteins from distantly related bacteria Article (PDF Available) in Journal of Bacteriology (1) In Escherichia coli this class of proteins is exemplified by the sensory transducers (also called signalling proteins or methyl-accepting chemotaxis proteins) which have a central role in mediating chemotactic behaviour 2,3.

The sensory transducers are the products of four genes: tsr, tar, tap and trg. Limited homology between trg and the other transducer proteins of Escherichia coli. J Bacteriol. Dec; (3)– [Europe PMC free article] [Google Scholar] Harayama S, Engström P, Wolf-Watz H, Iino T, Hazelbauer GL.

Cloning of trg, a gene for a sensory transducer in Escherichia coli. J Bacteriol. Oct; (1)–   Purification of receptor protein Trg by exploiting a property common to chemotactic transducers of Escherichia coli. J Biol Chem.

Oct 15; (29)– [Google Scholar] Chelsky D, Dahlquist FW. Structural studies of methyl-accepting chemotaxis proteins of Escherichia coli: evidence for multiple methylation sites.

The chemotactic signal transduction pathway of E. coli includes two high-abundance (or major) receptors, Tsr and Tar, as well as three low-abundance (or minor) receptors, Trg.There dre four MCP species in E. coli (Tar, Tap, Trg, and Tsr), each of which mediates responses to a different set of chemoeffector compounds.

Mutations that inactivate one of these transducers eliminate only a subset of chemotactic responses (Harayama et al., ; Parkin.Limited homology between trg and the other transducer proteins of Escherichia coli. J Bacteriol. Dec; (3)– [Europe PMC free article] [Google Scholar] Fox GE, Stackebrandt E, Hespell RB, Gibson J, Maniloff J, Dyer TA, Wolfe RS, Balch WE, Tanner RS, Magrum LJ, et al.

The phylogeny of prokaryotes. Science.